Video 5. Fibroblast contamination.

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Human Schwann cells (hSCs) were plated in LP medium and imaged via IncuCyte ZOOM^TM every 30 min. This culture contained a high proportion of fibroblasts, which can be recognized by their expanded (low contrast) cytoplasm and conspicuous nuclei. The hSCs extended processes but failed to align with one another, possibly due to the presence of fibroblasts concentrating in the central area. This culture has abundant cellular debris. The over-proliferation of fibroblasts and the debris can signal an underlying issue with the hSC culture (see Table 4). Some relevant elements were highlighted in the video. For a reference, compare this video with Video 1, which displays a pure hSC culture under the same culture conditions.