| Reagent [stock concentration] | Final concentration | Volume |
| DMEM/F12 | n/a | 45 mL |
|
FBS (100%)
GlutaMax 200 mM (100×)
HEPES 1 M (100×)
Gentamycin 50 mg/mL (1,000×)
Total
|
1%
1×
1× (10 mM)
1×
n/a
|
0.5 mL
0.5 mL
0.5 mL
0.05 mL
50 mL
|
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