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(a) Scheme of TFL incorporation and CCM encapsulation to generate Q2TFL and Q2TFL•CCM (b) of Q2TFL protein (6.97 kDa) after expression. L: Ladder, Pre: Preinduction with IPTG, Post: Postinduction with IPTG. (c) Q2TFL protein after purification. L: Ladder, FT: Flow-through, following are increasing concentrations of imidazole. (d) Representative MALDI-TOF spectra showing incorporation of TFL into Q2 by the size increase to 6.97 kDa.
