Figure 4.

(a) NMR spectrum at 500 MHz (11.7-T) of Q2_TFL at 1.5 mM shows two peaks (magenta and purple arrows), (b) SNR of Q2_TFL and Q_TFL as a function of protein concentration. (c) Temperature dependence of SNR from independent peaks. (d) Linear correlation of temperature with SNRT ratio showing the ability to predict temperature from ¹⁹F MRS. (e) Linear correlation of temperature with average (n = 3) fraction folded of Q2_TFL as assessed by CD. (f) Linear correlation of average fraction folded (n = 3) as assessed by CD with SNRT ratio showing ability to predict relative structure from ¹⁹F MRS.