Figure 6.

Ultrasound guided injection imaging: (a) Sagittal view of the hindlimb right before the needle insertion which is adequately tilted at 45° to expose to joint and ease the infusion. (b) The needle insertion within the hindlimb knee joint. (c) Successful injection of Q2_TFL into the hindlimb knee joint appearing as an echogenic signal using high frequency ultrasound. Red arrow indicates the syringe tip, and blue arrows indicate the presence of Q2_TFL. (d) 3D rendering of ¹H MRI imaging of the mouse hindlegs where Q2_TFL fibers (highlighted in green) appeared as a hypointense signal in the 3D MRI data sets in the injected hindleg. (e) ¹⁹F MR spectroscopy performed in vivo after injection of Q2_TFL using 10 min scan (TR = 100 ms, N_AV = 6000).