Figure 4.
Comparison of solubility, human tumor cell to plasma partitioning of DRP-104 and P11, and their antiproliferative effect. (A) Aqueous solubility of DRP-104 and P11 in buffer at pH 7.4. (B) Human tumor cell to plasma partitioning of DRP-104 and P11 were conducted by incubating either compound for 1 h in P493B lymphoma cells suspended in human plasma. Both intact DRP-104 and P11 levels were measured in human plasma and tumor cells, as well as prodrug-derived DON release in human plasma and tumor cells. (C) Cell viability assay was performed using P493B lymphoma cells incubated with DRP-104 and P11 for 72 h. Nonlinear regression analysis of the log-transformed data gave EC50 values.