Fig. 6.

miR-21 inhibits PTEN expression in LR-MSCs. A qRT‒PCR analysis of miR-21 expression with the addition of miR-21 inhibitor or mimics in LR-MSCs. B, C qRT‒PCR and WB analysis of PTEN expression with the addition of miR-21 inhibitor or mimics in LR-MSCs. D Construction of the luciferase reporter system. E Dual-luciferase reporter assay to validate the direct binding between miR-21 and the PTEN 3′-UTR. F RIP-qPCR analysis of miR-21 and its interacting 3′ UTR of PTEN enrichment by Ago2. G, H The mRNA and protein expression levels of PTEN in LR-MSCs with or without TGF-β1 treatment and miR-21 inhibitor. β-Actin was used as a reference gene. **P < 0.01