TABLE 1.

Recovery of T. vaginalis from mouse vaginal washes^e

Groupa	Dose or treatmenta	No. of mice with T. vaginalis on the indicated day of culture/total no. inoculated
		+7	+14	+21	+28
1	4.5 × 105 organisms/ml (n = 26)	12/26	10/25b	8/25b,c	7/25b,c
2	9 × 106 organisms/ml (n = 26)	6/25b,c	5/25b,c	3/24b,c,d	1/24b,c,d
3	1 × 108 organisms/ml (n = 26)	6/25b,c	4/23b,c	1/21b,c,d	0/21b,c,d
4	PBS (n = 18)	16/17b	13/16b	9/15b	8/14b
5	No immunization (n = 18)	14/16b	12/16b	10/15b	7/15b
6	No treatment (negative control) (n = 6)	0/6	0/6	0/6	0/6

^aAll mice in groups 1 to 5 received 0.05 ml of estradiol valerate subcutaneously on days −9 and −2, vaginal inoculation with 10¹⁰ L. acidophilus organisms per ml on days −7 and −6, and vaginal infection with 2.5 × 10⁷ T. vaginalis organisms per ml. Groups 1 to 4 received subcutaneous inoculation of T. vaginalis on days −56 (in Freund’s complete adjuvant) and −28 (in Freund’s incomplete adjuvant) in the doses indicated. PBS, phosphate-buffered saline. Group 6 received none of the above manipulations. 

^bTotal does not equal n because of unrelated death of mice. 

^cStatistically significant compared with group 4 value. 

^dStatistically significant compared with group 1 value. 

^eReprinted from reference 3 with permission of the publisher.