TABLE 3.
Staining characteristics of selected bacterial genera
| Genus | Staining witha:
|
Other techniques | |||||
|---|---|---|---|---|---|---|---|
| PAP | Romanowsky | H&E | Gram | Acid fast | Silver | ||
| Mycobacterium | − | + | − | − | ++ | GMS | PCR |
| (negative images) | |||||||
| Legionella | − | +b | − | + | −c | Dieterle, W-S | DFA |
| modified | |||||||
| Actinomyces | + | + | + | + | − | GMS | DFA |
| Helicobacter | + | + | + | + | − | Dieterle, W-S | |
| Nocardia | − | +b | − | + | +d | GMS | DFA |
a
DFA, direct fluorescent antibody; W-S, Warthin-Starry; −, does not stain; +, visible with stain.
b
Stains best with Giemsa.
c
L. micdadei will stain acid-fast positive.
d
Stains best with Fite’s silver stain.