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. 2023 Nov 28;223(2):e202211026. doi: 10.1083/jcb.202211026

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© 2023 Mahone et al.

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Figure S3. — ZapA localization reflects Z-ring position in filamentous cells depleted of FzlA, and FtsK and FzlA localize independently of each other and of FtsW. (A) Phase-contrast, epifluorescence, and merged images of a strain expressing zapA-mNG dependent on xylose for production of FzlA (EG3523). The strain was either continuously induced (top) or depleted for 7 h (bottom) by being grown in the presence of 0.001% xylose or glucose, respectively. (B) Representative phase-contrast, epifluorescence, and merged images of ftsk-mCherry at its native locus in a WT (EG1291), ftsW**I* (EG3926), or ftsW**I* ∆fzlA (EG2481) background, and in an FtsW depletion strain (EG740). The FtsW depletion strain was grown in the presence (+FtsW) or absence (−FtsW) of 0.3% xylose for 20 h and then imaged. (C) Representative phase-contrast, epifluorescence, and merged images of vanillate inducible mCherry-fzlA in either an FtsK_FL (EG3935) or FtsW (EG3928) depletion strain. The FtsK_FL depletion strain was grown in the presence (+FtsK_FL) or absence (−FtsK_FL) of 0.3% xylose for 20 h before imaging. The FtsW depletion strain was grown in the presence (+FtsW) or absence (−FtsW) of 0.3% xylose for 14 h before imaging. mCherry-fzlA production was induced in both strains by adding 0.5 mM of vanillate for 2 h before imaging. Scale bars are all 2 µm.