Table 3. Estimated sensitivity to detect STH infection by assay method in a single infected child for different assumed intensity infections (for fecal testing methods used in a STH prevalence study conducted in Lowndes, Wilcox, and Perry Counties, Alabama, USA, December 2019–August 2022*.
| Egg/g feces from 1 child |
Mini-FLOTAC, triplicate, % |
qPCR, single, % |
dPCR, single, % |
Combined, % |
| Sensitivity accounting for recovery | ||||
| 1 | 2 | 2 | 18 | 20 |
| 3 | 5 | 6 | 53 | 55 |
| 5 | 9 | 10 | 89 | 90 |
| 7 | 12 | 14 | 100 | 100 |
| 10 | 17 | 20 | 100 | 100 |
| 100 |
93 |
100 |
100 |
100 |
| Sensitivity not accounting for recovery | ||||
| 1 | 2 | 10 | 90 | 91 |
| 3 | 7 | 30 | 100 | 100 |
| 5 | 12 | 51 | 100 | 100 |
| 7 | 17 | 71 | 100 | 100 |
| 10 | 23 | 100 | 100 | 100 |
| 100 | 99 | 100 | 100 | 100 |
*dPCR, digital PCR; qPCR, quantitative PCR.