Table 5. Antibodies.
| Antibody | Company | Catalogue number | Antigen | Dilution IF | Dilution WB |
|---|---|---|---|---|---|
| α-Tubulin | Abcam | ab7291 | 1:10,000 in 3% BSA | ||
| α-Tubulin | Merck Millipore (Billerica, MA, USA) | 16-232 | 1:1000 | ||
| CapZa3 | Progen | GP-SH4 | 1:500 | ||
| Ccin | Progen | GP-SH3 | 1:500 | ||
| Cylc1 (used for mouse) | Davids Biotechnology (Regensburg, Germany) | Custom-made polyclonal antibody | AESRKSKNDERRKTLKIKFRGK and KDAKKEGKKKGKRESRKKR |
1:1000 (sperm cells) 1:500 (testis tissue) 1:1000 western blot |
1:1000 in 5% milk in TBST |
| Cylc1 (used for human samples) | Santa Cruz | sc-166400 | 1:500 | ||
| Cylc2 | Davids Biotechnology (Regensburg, Germany) | Custom-made polyclonal antibody | KSVGTHKSLASEKTKKEVK and ESGGEKAGSKKEAKDDKKDA | 1:1000 (sperm cells) 1:500 (testis tissue) 1:1000 western blot |
1:1000 in 5% milk in TBST |
| Hook1 | Proteintech | 10871-1-AP | 1:500 | ||
| PLCζ | Invitrogen | PA5-50476 | 1:100 | ||
| Sp56 | Invitrogen | MA1-10866 | 1:500 |