Table 1.
Primers used for the 5ʹ/3ʹ RACE.
| Virus | Primer | Sequence (5ʹ-…-3ʹ) |
|---|---|---|
| PipaV | SP1 | TACTGCATTTTTTGACCCGTAC |
| SP2 | GACATCAACAAGAAAAAGAAATGAGG | |
| SP3 | GGTCTCAAAGAGGTATCAACAATACC | |
| SP4 | GAGTGAAGAAGGCTGCAG | |
| SP5 | GGATCCAGGGGACGCTGATG | |
| NinYsV | SP1 | GGGTATGCTTTAAATCTGACTTGG |
| SP2 | CCAGGATTATCTTCTTAGGAGATT | |
| SP3 | ACCTGGTCATACGAATTGTCCAC | |
| SP4 | TTTTCTTGGGTCTTTCATTA | |
| SP5 | GCAATTGAGTTGATAACCCTCCC | |
| General | 5ʹ oligo d(G)-anchor | GACCACGCGTATCGATGTCGACGGGGGGGGGGGGGGACC |
| 3ʹ oligo d(T)-anchor | GACCACGCGTATCGATGTCGACTTTTTTTTTTTTACC | |
| PCR anchor | GACCACGCGTATCGATGTCGAC |