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. 2023 Nov 21;2023:4193309. doi: 10.1155/2023/4193309

Figure 4.

Figure 4

Rapamycin decreases high-glucose injury in HK-2 cells.(a)Flow cytometry and quantification to assay apoptosis of HK-2 cells under the NG, HG, HG plus treatment with a plasmid expressing an shRNA against Netrin-1 (shNetrin-1), HG plus rapamycin (a mTOR inhibitor) and HG+Netrin-1+Rapa. (b) Western blotting analyses of Bcl-2, Bax and cleaved caspase-3 in HK-2 cells. Equal protein loading was confirmed using an anti-GAPDH antibody; Band intensity of Bcl-2, Bax and cleaved caspase-3. (c) Fluorescein—phalloidin staining of actin fibers with indicated treatments and transfections (scale bar = 20 μm). Values are means ± SEMs. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. vs. HG group: ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001. vs.HG+Rapa group: &P < 0.05, &&P < 0.01, and &&&P < 0.001.