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. 2023 Sep 25;89(15):1493–1504. doi: 10.1055/a-2173-8627

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The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. ( https://creativecommons.org/licenses/by-nc-nd/4.0/ )

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.

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Fig. 4 — Analysis and sequencing of kalata S. a Analytical HPLC trace of kalata S. b MALDI-MS spectrum of kalata S. c Annotated MS/MS spectrum of the m/z 3243.14 precursor obtained after proteolytic cleavage with endoproteinase Glu-C in the range m/z 0 – 1000 (top), m/z 1000 – 2000 (middle), and m/z 2000 – 3300 (bottom). Additionally, the sequence of kalata S is shown on top and identified b- and y-ions are indicated. A list of all identified fragment ions is given in Table 1S , Supporting Information.