FIG. 3.

(A) Colony morphology of K60 transformed for 48 and 72 h with pepsR, pGepsRD-A, and pLAFR3. (B) Overexpression of EpsRD-A does not affect expression of the epsI promoter as measured in strain S49, containing the epsI::lacZ fusion. Cells containing the plasmids indicated at the bottom of the graph were grown to an OD₆₀₀ of 1.2 and assayed for β-galactosidase activity (see legend to Fig. 1 for clarification of values) as described in Materials and Methods.