TABLE 2.
Transformation phenotypes of comG mutants
| Strain | Relevant genotype | Protein affected | Missing residuesa | % Transformationb |
|---|---|---|---|---|
| BD630 | Wild type | None | None | 0.13 |
| BD2685 | comGΔA | ComGA | 162 (356) | <10−6 |
| BD2686 | comGΔB | ComGB | 167 (323) | <10−6 |
| BD1770 | comGΔC | ComGC | 14 (98) | <10−6 |
| BD1260 | comGC107 | ComGCDEFG | None | <10−6 |
| BD2684 | comGC107 amyE::comGCDEFG | None | None | 0.09 |
| BD2687 | comGC107 amyE::comGC(ΔD)EFG | ComGD | 14 (143) | <10−6 |
| BD2688 | comGC107 amyE::comGCD(ΔE)FG | ComGE | 15 (115) | <10−6 |
| BD2689 | comGC107 amyE::comGCDE(ΔF)G | ComGF | 15 (165) | <10−6 |
| BD1252 | comGG210 | ComGG | None | <10−6 |
a
The numbers in parentheses refer to the total number of residues in the proteins.
b
Transformation frequency was scored as the number of leucine prototrophs per milliliter times 100 divided by the total CFU per milliliter.