TABLE 3.
DNA binding by comG mutants
| Strain | Relevant genotype | Protein affected | Radioactivity bound, cpm (%)a |
|---|---|---|---|
| BD630 | Wild type | None | 389 (100) |
| BD2685 | comGΔA | ComGA | 0 (0) |
| BD2686 | comGΔB | ComGB | 5 (1.3) |
| BD1770 | comGΔC | ComGC | 1 (0.25) |
| BD2684 | comGC107 amyE::comGCDEFG | None | 261 (67) |
| BD2687 | comGC107 amyE::comGC(ΔD)EFG | ComGD | 0 (0) |
| BD2688 | comGC107 amyE::comGCD(ΔE)FG | ComGE | 0 (0) |
| BD2689 | comGC107 amyE::comGCDE(ΔF)G | ComGF | 0 (0) |
| BD1252 | comGG210 | ComGG | 0 (0) |
a
Radiolabeled DNA was incubated with cultures grown to competence. After washing, the total radioactivity associated with the cells in a fixed volume of culture was determined. The numbers in parentheses refer to percent values normalized to the wild-type level. The zero values indicate that the radioactivity determined was less than background but not by more than 5 cpm.