Fig. 9. Comparative distribution analysis of aGVHD grading systems refined beyond four grades on the independent test cohort (n = 700).

Additional data-driven aGVHD gradings with more than 4 grades are compared to reveal differences in patient proportions, organ combinations involved in each grade and their ability to dissect into cohorts with significantly distinct NRM. a Cumulative incidence NRM curves of aGVHD grades in PC1 grading using all 12 PC1 stages as distinct severity grades. Colors representing PC1 aGVHD grade I–XII. Separation of curves is tested by the two-sided Gray test. b Pie chart according to PC1 with 12 grades. The angle of each slice represents the number of organ stage combinations in the respective grade. The radius of each slice represents the number of patients within this grade. c Stacked bar chart of PC1 with 12 grades showing the proportion of patients in each organ stage combination. For each bar, one color represents one combination, no crossover between grades. The phenotypes are detailed in Supplementary Data 9. d Cumulative incidence NRM curves according to PC1 grading with 6 grades. Colors representing PC1 aGVHD grade I–VI. Separation of curves is tested by the two-sided Gray test. e Pie chart of PC1 with six grades. f Stacked bar chart of PC1 with six grades. The phenotypes are detailed in Supplementary Data 10. g Cumulative incidence NRM curves according to K-means grading with eight grades, using the optimal number of clusters as determined by elbow method on the development cohort. Colors representing K-means aGVHD grade I–VIII Separation of curves is tested by the two-sided Gray test. h Pie chart of K-means-8 grades i Stacked bar chart of K-means-8 grades. The phenotypes are detailed in Supplementary Data 11. Source data for b, c, e, f, h and i are provided as a source data file.