Table 2.
Accuracy and precision obtained from the analysis of human plasma quality control samples spiked with bosentan and its metabolite analysed by LC with enhanced product ion mode CID/UVPD and UVPD. LOQQC limit of quantification QC, LQC low QC, MQC medium QC, HQC high QC used for calibration range 5 to 500 ng/mL
| Amount added (ng/mL) | Bosentan (CID + UVPD) | Desmethyl bosentan (UVPD) | n | |||
|---|---|---|---|---|---|---|
| CV (%) | Accuracy (%) | CV (%) | Accuracy (%) | |||
| LOQQC2 | 5 | 16.7 | 112 | 7.6 | 116 | 5 |
| LQC2 | 15 | 11.1 | 108 | 7.5 | 95.8 | 5 |
| MQC2 | 50 | 9.2 | 99.8 | 7.6 | 91.1 | 5 |
| HQC2 | 500 | 5 | 112 | 4.8 | 121 | 5 |