Table 1.
Primers used for analysis of the expression of ribonucleotide reductase genes (rnr1 and rnr2) and actin-lateral binding (ALb) gene in chlamydospores of Fusarium oxysporum f. sp. cubense Tropical Race 4 (Cohen et al. 2019)
| Target gene | Primer name | Sequence (5ʹ → 3ʹ) |
|---|---|---|
| rnr1 (F. oxysporum ribonucleotide reductase large subunit) | rnr1-F | CATCAAGGCTGATGTTGAGG |
| rnr1-R | CTTGACACCCAACTCTTCTTCC | |
| rnr2 (F. oxysporum ribonucleotide reductase small subunit) | rnr2-F | TACTTTGGATCCGGAAGCTG |
| rnr2-R | TTTTCATCCACCCTGAGTCC | |
| ALb (F. oxysporum actin-lateral binding protein) | ALb-F | GGTTTCCCTTCAGCCTTTTC |
| ALb-R | CGGAGCTGGTTCATTTTCTC |