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In vivo expression of the two B. anthracis S-layer components. Immunoblotting of pellet fractions (odd-numbered lanes) and supernatant fractions (even-numbered lanes) of S-layers from wild-type (CAF10) (lanes 1 and 2), Δsap (CBA91) (lanes 3 and 4), Δeag (CSM91) (lanes 5 and 6), and Δeag Δsap (CSM11) (lanes 7 and 8) strains was carried out with pooled sera from mice infected with the CAF10 strain. Molecular masses are indicated in kilodaltons on the left.
