TABLE 1.
B. anthracis strains and relevant plasmids used in this study
| Strain or plasmid | Relevant characteristics | Phenotype or markera | Reference or source |
|---|---|---|---|
| B. anthracis strains | |||
| CAF10 | pXO1− pXO2+ | Cap+ EA1+ Sap+ | 8 |
| CBA91 | Δsap derivative of CAF10 via allelic exchange between pEAI207 and CAF10 chromosome | Cap+ EA1+ | This study |
| CSM91 | Δeag derivative of CAF10 via allelic exchange between pSAL322 and CAF10 chromosome | Cap+ Sap+ | This study |
| CSM11 | Δsap Δeag derivative of CAF10 via allelic exchange between pSAL303 and CAF10 chromosome | Cap+ | This study |
| Plasmids | |||
| pEAI207 | Conjugative suicide plasmid carrying deleted sap gene | Err Knr Spr | 6 |
| pSAL322 | Conjugative suicide plasmid carrying deleted eag gene | Err Knr Spr | 20 |
| pSAL303 | Conjugative suicide plasmid carrying deleted eag and sap genes | Err Knr Spr | 20 |
a
Err, Knr, and Spr, resistance to erythromycin, kanamycin, and spectinomycin, respectively.