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. 2023 Nov 17;4(4):102709. doi: 10.1016/j.xpro.2023.102709

Figure 2.

Figure 2

Experimental setup with all control and sample tubes used

(A) Patient-derived neuroblastoma cell line ST16-4045 was used for single-stain compensation controls, as were UltraComp eBeads PLUS. A control bone marrow sample from a B-ALL patient during remission (with or without spike-in of ST16-4045) was stained with the entire 9-color antibody panel, as were CHO cells (a negative control) and neuroblastoma cell lines (used as benchmark controls for antigen density). Experimental samples were stained with the full antibody panel.

(B) The staining procedure involves pre-incubating samples with Fc Block. It is important to use Brilliant Stain Buffer and to add the additional BV antibodies separately, to avoid the antibodies binding to each other instead of the cells.