REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Human CD13-BUV737 (clone L138, 1:40)	BD Biosciences	Cat# 749265
Human CD45-PerCp-Cy5.5 (clone HI30, 1:25)	Thermo Fisher Scientific	Cat# 45-0459-42
Human GPC2 (clone D3, PE labeled, 1:200)	Dr. Dimiter S. Dimitrov (University of Pittsburgh)	N/A
Human GD2-BV510 (clone 14g2a, 1:20)	BioLegend	Cat# 357316
Human NCAM-BV605 (clone 5.1H11, 1:100)	BioLegend	Cat# 362538
Human L1CAM-BV421 (clone 5G3, 1:200)	BD Biosciences	Cat# 565732
Human L1CAM-PE (clone 5G3, 1:200)	BD Biosciences	Cat# 564193
Human ALK (clone 48, APC labeled, 1:500)	Mark Vigny	N/A
Human B7-H3-PE-Cy7 (clone MIH42, 1:40)	BioLegend	Cat# 351008
Biological samples
Human neuroblastoma-infiltrated bone marrow samples	Stanford Pediatrics Bass Center Tissue Bank (IRB: #45458 and #56619)	N/A
Human bone marrow samples from B-ALL patients (uninvolved tumor-free at remission)	Stanford Pediatrics Bass Center Tissue Bank (IRB: #45458 and #56619)	N/A
Chemicals, peptides, and recombinant proteins
Abcam PE Conjugation Kit Lightning-Link	Abcam	Cat# ab102918
Abcam APC Conjugation Kit Lightning-Link	Abcam	Cat# ab201807
BD Quantibrite PE beads	BD Biosciences	Cat# 340495
BD custom quantitation beads in APC	BD Biosciences	Custom 626425
BD custom quantitation beads in PE-Cy7	BD Biosciences	Custom 625639
BD custom quantitation beads in BV421	BD Biosciences	Custom 625508
BD custom quantitation beads in BV510	BD Biosciences	Custom 625594
BD custom quantitation beads in BV605	BD Biosciences	Custom 625638
UltraComp eBeads Plus compensation beads	Thermo Fisher Scientific	Cat# 01-3333-41
Experimental models: Human cell lines and PDX
CHO-K1 (female)	ATCC	RRID:CVCL_0213
NGP (male)	Dr. John Maris (CHOP)	RRID:CVCL_2141
NGP-NPC2 (male)	Dr. John Maris (CHOP)	N/A
SMS-SAN (female)	Dr. John Maris (CHOP)	RRID:CVCL_7136
NBSD (female)	Dr. John Maris (CHOP)	RRID:CVCL_LF68
ST16-BM4045 (male, diagnostic)	This manuscript	N/A
Software and algorithms
FlowJo v10.7.1	BD Biosciences	https://www.flowjo.com/solutions/flowjo/downloads
GraphPad Prism v8.4	GraphPad Software, Inc.	https://www.graphpad.com/updates
BioRender	BioRender	https://www.biorender.com
Other
LSRFortessa X-20 4 lasers (405 nM violet, 488 nM blue, 640 nM red, and 355 nM UV)	BD Biosciences	N/A
Fixable viability dye eFluor 780	Thermo Fisher Scientific (eBioscience)	Cat# 65-0865-14
Brilliant stain buffer	BD Biosciences	Cat# 566349
Human BD Fc Block	BD Biosciences	Cat# 564219
Corning Cellstripper dissociation reagent	Corning	Cat# MT25056CI
Ficoll-Paque PLUS density gradient media	Cytiva	Cat# 17144002
Gibco DPBS, no calcium, no magnesium	Thermo Fisher Scientific	Cat# 14190144
Invitrogen EDTA (0.5 M), pH 8.0, RNase-free	Thermo Fisher Scientific	Cat# AM9260G
Fetal bovine serum, qualified, heat inactivated	Thermo Fisher Scientific	Cat# 16140071
Corning Falcon round-bottom polystyrene test tubes with cell strainer snap cap	Fisher Scientific	Cat# 08-771-23
Nalgene Mr. Frosty freezing container	Thermo Fisher Scientific	Cat# 5100-0001
Corning Costar 96-well, cell culture treated, U-shaped-bottom microplate	Fisher Scientific	Cat# 07-200-95
Corning Falcon 15 mL conical centrifuge tubes	Fisher Scientific	Cat# 14-959-49D
RPMI 1640 medium, no glutamine	Thermo Fisher Scientific	Cat# 21870092