FIGURE 2.

Oocyte Morphology after 24 h in vitro maturation with anti-miR-21 LNAs and BPA. Bovine COCs were placed into maturation media alone (A–C), in the presence of a scramble (D–F), or in the presence of anti-miR-21 LNA inhibitors (G–I) at 0.5 µM. 12 h into maturation, COCs were also treated with either a vehicle (B,E,H) or BPA (C,F,I) at 0.05 mg/mL for another 12 h. BPA-treated COCs appear poorer in quality with less cumulus cell expansion and increased heterogeneity among COC phenotypes. Scale bar = 500 µm.