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Corrected Total Cell Fluorescence of 5′hydroxymethylcytosine in Bovine Oocytes. Transfected and treated COCs were fixed and permeabilized to allow for immunostaining of 5 hmC. The corrected total cell fluorescence was calculated using ImageJ on a minimum of 15 COCs per group for a total of 140 COCs analyzed. Bars represent the mean ± SEM at p < 0.05.
