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. 1998 Jan;180(1):65–72. doi: 10.1128/jb.180.1.65-72.1998

FIG. 2.

FIG. 2

Complementing cosmids and plasmids of SMEG 23. (A) Agarose gel electrophoresis of PvuII digestions of 10 cosmids recovered from temperature-resistant transformants of SMEG 23. Cosmid pAEB205 is shown in lane 8. Sizes of DNA markers (M) are shown in kilobase pairs. (B) Agarose gel electrophoresis of PvuII digestions of plasmid subclones derived from cosmid pAEB205 DNA. Plasmids pAEB209, pAEB207, pAEB210, and pAEB208 are shown in lanes 2, 5, 8, and 9, respectively. (C) Restriction map of cosmid pAEB205 and plasmids derived from it with their corresponding complementation of SMEG 23. Relevant restriction sites for PvuII (P), EcoRV (E), ClaI (C), HindIII (H), and NheI (N) are shown. The positions of the 0.6-, 4.2-, and 1.8-kb PvuII fragments are shown. Thick vertical bars indicate the junctions between the insert DNA and vector DNA (not shown).