FIG. 3.
Organization of the dnaG locus in M. smegmatis. The open reading frames identified within a 5.1-kb segment of M. smegmatis DNA are shown as arrows, and the tRNAAsn gene is shown as an arrowhead. ORF1 and ORF5 extend beyond the segment of DNA for which the DNA sequence was determined. The signals for expression of both the divergently transcribed ORF1 and dgt (and dnaG) most likely lie within the 59-bp intergenic region between the start codons for ORF1 and dgt. The dgt gene is located immediately upstream of dnaG. There are 53 bp between the stop codon of ORF2 and the start codon of dnaG. There is a sequence capable of forming a possible RNase III site in the 430-bp intergenic region between ORF4 and the tRNAAsn. Relevant enzyme sites are shown. The position of the mutation conferring the temperature-sensitive phenotype in SMEG 1 is indicated by an asterisk.