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. 2023 Nov 2;31:100743. doi: 10.1016/j.omto.2023.100743

Figure 3.

Figure 3

Combined virus SV5 generates larger plaques than T3wt in breast cancer cell lines

(A) Representative images illustrating the plaque sizes generated by T3wt and SV5 in the breast cancer cell lines MCF7, T47D, and TUBO. (B) Graph showing plaque size of single mutated viruses and SV5 relative to T3wt in MCF7, T47D, and TUBO cells. Each point represents an independent experiment performed in duplicate. Boxes represent the average plaque size ± standard error of three or more independent experiments relative to T3wt (n = 3–8). Cells were infected with T3wt, single mutated viruses, and SV5. MCF7 and TUBO cells were fixed after 5 days of infection. T47Ds were fixed after 8 days. Immunocytochemistry with a polyclonal anti-reovirus antibody was performed in MCF7 and TUBO cells to detect plaques, while T47D cells were stained with crystal violet. ∗∗∗p < 0.001; ns, non-significant (ANOVA with Tukey test).