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. 1998 Jan;180(1):73–82. doi: 10.1128/jb.180.1.73-82.1998

FIG. 8.

FIG. 8

Primer extension analysis of the 5′ ends of 5S, 16S, and 23S rRNA in wild-type and mutant strains. Lanes 1, strain MW38 (rimM+ rbfA+); lanes 2, strain MW37 (ΔrimM-2 rbfA+); lanes 3, strain PW109 (ΔrimM-2 sdr-43 rbfA+); lanes 4, strain GOB162 (rimM+ rbfA::Kmr). The sizes of the primer extension products obtained were determined by comparing the mobilities with those of a known DNA sequencing ladder. (A) RIII indicates a primer extension product of 179 nt corresponding to pre-16S rRNA processed at the RNase III site 115 nt upstream of the 5′ end of mature 16S. M indicates a product of 64 nt corresponding to the 5′ end of mature 16S rRNA. (B) M indicates a primer extension product of 63 nt corresponding to mature 5S rRNA. (C) RIII indicates a primer extension product of 65 nt corresponding to pre-23S rRNA processed at the RNase III site 7 nt upstream of the 5′ end of mature 23S. M indicates a product of 58 nt corresponding to the 5′ end of mature 23S rRNA.