Table- 3.

P. aeruginosa gene expression in exposure to bile.

Gene symbol	Function/presence	Regulation/Expression in response to bile	Technique	Bile/bile salt	Concentration	Strain	Ref.
mqoB	Glyoxylate shunt pathway	Increase	Real-time PCR	Bile	0.30 %	PAO1	[50]
glcB	Glyoxylate shunt pathway	Increase	Real-time PCR	Bile	0.30 %	PAO1	[50]
acnA	The early stage of the TCA cycle	Increase	Real-time PCR	Bile	0.30 %	PAO1	[50]
idh	The early stage of the TCA cycle	Increase	Real-time PCR	Bile	0.30 %	PAO1	[50]
PA0853-54	The late stages of the TCA cycle	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]
aspA	The late stages of the TCA cycle	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]
lpdV	glucose metabolism/glycolysis	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]
gapA	Glucose metabolism/glycolysis	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]
glk	Glucose metabolism/glycolysis	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]
aceEF	Glucose metabolism/glycolysis	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]
pfm	Proton motive force	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]
pqsA	Biofilm formation and virulence	Increase	Real-time PCR	Bile	0.30 %	PAO1	[50]
pqsA-E	QS/biofilm formation and virulence	Increase	Promoter fusion and thin-layer chromatography	Bile	0.30 %	PA14, PAO1 and clinical isolates CF242	[27]
rhlI	Acyl-homoserine-lactone synthase	Increase	Promoter fusion and thin-layer chromatography	Bile	0.30 %	PA14, PAO1 and clinical isolates CF242	[27]
lasI	Acyl-homoserine-lactone synthase	Increase	Promoter fusion and thin-layer chromatography	Bile	0.30 %	PA14, PAO1 and clinical isolates CF242	[27]
psl	Biofilm formation and virulence	Increase	Congo red binding assay	Bile	0.30 %	PAO1	[50]
psrA	Transcriptional regulator associated with biofilm formation	Increase	Real-time PCR	Bile	0.30 %	PAO1	[50]
ppyR	Transcriptional regulator associated with iron-scavenging and exopolysaccharide production	Increase	Real-time PCR	Bile	0.30 %	PAO1	[50]
ohrR	Transcriptional regulator associated with oxidative stress response	Increase	Real-time PCR	Bile	0.30 %	PAO1	[50]
mexR	Transcriptional regulator associated with secretion and antibiotic tolerance	Increase	Real-time PCR	Bile	0.30 %	PAO1	[50]
T3SS	Type III secretion systems (associated with acute infection)	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]
T3SS effector exoU	Promoter activity of the T3SS effector exoU	8.9- fold decrease	Real-time PCR	Bile	0.30 %	PA14, PAO1 and clinical isolates CF242	[27]
T6SS	Type vI secretion systems	Decrease	Real-time PCR	Bile	0.30 %	PA14, PAO1 and clinical isolates CF242	[27]
hcnABC	Hydrogen cyanide	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]
amiR	Amidase	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]
phzA-E	Phenazine	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]
gnyR	Transcriptional regulator associated with isoprenoid degrading	Decrease	Real-time PCR	Bile	0.30 %	UCBPP-PA14, PA14_38430	[50]
sfa2	Transcriptional regulator associated with T6SS HSI-II expression	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]
mexAB-OprM	Efflux pump/Antibiotic tolerance	Increase	Real-time PCR	Bile	0.30 %	PAO1	[50]
SiaA-D	Transition to a sessile lifestyle through cyclic-di-GMP synthesis	Upregulated	Real-time PCR	Bile	0.30 %	PAO1	[50]
phzS	PYO biosynthetic genes	Increase	qRT-PCR	Bile	0.30 %	PA14	[31]
phzH	PYO biosynthetic genes	No significant response	qRT-PCR	Bile	0.30 %	PA14	[31]
phzM	PYO biosynthetic genes	No significant response	qRT-PCR	Bile	0.30 %	PA14	[31]
gdbR	Transcriptional regulator associated with choline	Decrease	Real-time PCR	Bile	0.30 %	PAO1	[50]