Table 2. Parameters for Tanaka Test on Sil-Ala-C12(SIL) and Commercial HILIC Columnsa.
| property tested | SIL | Luna-Diol | ZIC-HILIC | Luna-NH2 |
|---|---|---|---|---|
| hydrophilicity of the phase (k (U)) | 0.41 | 0.65 | 3.12 | 2.76 |
| hydrophobicity/methylene selectivity (α(CH2)) | 1.03 | 1.17 | 1.69 | 1.29 |
| hydroxyl group selectivity (α(OH)) | 3.58 | 1.35 | 2.02 | 1.79 |
| configurational selectivity (α(V/A)) | 1.54 | 1.30 | 1.48 | 1.52 |
| regio selectivity (α(2′-dG/3′-dG)) | 1.02 | 1.04 | 1.11 | 1.06 |
| shape selectivity (α(NPαGlu/NPβGlu)) | 1.46 | 1.16 | 1.17 | 1.10 |
| anion exchange selectivity (AX) | 9.99 | 0.10 | 0.17 | 6.89 |
| cation exchange selectivity (CX) | 0.49 | 2.43 | 0.22 | 0.23 |
| pH on the surface of the stationary phase (α(Tb/Tp)) | 0.11 (basic) | 1.04 (neutral) | 1.16 (neutral) | 0.68 (basic) |
a
Chromatographic condition: mobile phase: Acetonitrile-ammonium acetate buffer (20 mM, pH 4.7) (90:10, v/v). Flow rate: 0.2 mL/min. Column temperature: 30 °C. UV detection: 254 nm. Injection volume: 5 μL.