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. 2023 Nov 29;21:457. doi: 10.1186/s12951-023-02187-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Characterization and drug loading capacity of GQDs/Cy5-miR@sEVs. A TEM images of sEVs (upper panel) and GQDs/Cy5-miR@sEVs (lower panel). Scale bar = 200 nm. B Size distribution of sEVs and GQDs/Cy5-miR@sEVs. C Expression of Alix, CD63, CD9, Calnexin in sEVs, GQDs/Cy5-miR@sEVs and HucMSCs was detected by Western blot. D MiR-193a-3p in GQDs/Cy5-miR@sEVs and sEVs were detected by qRT-PCR. E The degradation of Cy5-miR, GQDs/Cy5-miR and GQDs/Cy5-miR@sEVs in RNase solutions was detected by agarose gel electrophoresis. F Stability of GQDs/Cy5-miR and GQDs/Cy5-miR@sEVs in PBS in 50 min at room temperature. G Stability of GQDs/Cy5-miR and GQDs/Cy5-miR@sEVs in 50 min after being incubated within mouse serum at 37 °C. Data are expressed as mean ± SD and analyzed by one-way ANOVA. *p < 0.05, ***p < 0.001, ****p < 0.0001