Fitted parameters for the two-photon excitation time-correlated single photon counting decay curves at 910 nm excitation (1-BPin and 2-BPin solutions were 10 mM in DMSO and corresponding 1–2@β-d-glucan hybrid was in 1 : 1 DMSO : PB Buffer. Corresponding 2P FLIM data associated to micrographs of living HeLa cells depicted in Fig. 9, and ESI. The full width at half maximum (FWHM), calculated from the lifetime distribution curve within the focal area was used to assess the 2P lifetime distribution error.

2P TCSPC (λex 910 nm)							2P FLIM (λex 910 nm, 15 min in HeLa, peak ± error, ns)
	χ 2	τ 1 (ns)	a 1 (%)	τ 2 (ns)	a 2 (%)	τ m (ns)
1-BPin	1.24	0.41	98.9	5.94	1.1	0.48	1-BPin, 37 °C	2.86 ± 0.37 ns
							1-BPin, 4 °C	2.34 ± 0.23 ns
1@glucan	1.38	0.43	98.5	5.62	1.5	0.50	1@glucan, 37 °C	3.49 ± 0.48 ns
2-BPin	1.35	0.60	49.2	3.27	50.8	1.96	2-BPin, 37 °C	0.50 ± 0.07 ns
							2-BPin, 4 °C	1.65 ± 0.24 ns
2@glucan	1.46	0.89	41.2	4.47	58.8	2.99	2@glucan, 37 °C	1.23 ± 0.18 ns