TABLE 3.
Phenotypes of selected mutants
| Strain | Phenotypea | MIC (μg/ml)b
|
Accumulation
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amp | Cip | Chl | Ery | Tet | EtBr | AO | SDS | Deox | Bile | Cet | Tri | Cipc | EtBrd | ||
| P270 | WT | 4 | 0.25 | 1 | 0.25 | 0.25 | 4 | 16 | 8 | 16 | 64 | 8 | 4 | 15.9 ± 1 | 14.6 ± 1 |
| P1031 | Cipr | 2 | 32 | 1 | 0.25 | 0.25 | 4 | 16 | 8 | 16 | 64 | 8 | 4 | NDe | ND |
| P1048 | CmeB+ | 32 | 8 | 16 | 4 | 8 | 32 | 64 | 64 | 64 | 128 | 64 | 32 | 8.3 ± 2 | 7.4 |
| P1038 | CmeB− | 1 | 0.06 | 0.5 | 0.06 | 0.06 | 2 | 8 | 2 | 4 | 16 | 2 | 1 | 24.4 ± 2 | 21.7 ± 2 |
| P1040 | Cipr | 1 | 16 | 1 | 0.06 | 0.06 | 2 | 8 | 2 | 4 | 16 | 2 | 1 | ND | ND |
| P1035 | MAR | 1 | 4 | 4 | 0.06 | 0.06 | 8 | 32 | 8 | 4 | 16 | 2 | 1 | 19.8 ± 2 | 17.98 ± 2 |
| P1043 | CmeF− | 0.5 | 0.25 | 1 | 0.25 | 0.25 | 1 | 4 | 4 | 8 | 32 | 2 | 1 | 17 ± 1 | 23.2 ± 1 |
| P1045 | Cipr | 0.5 | 32 | 2 | 0.25 | 0.25 | 1 | 4 | 4 | 8 | 32 | 2 | 1 | ND | ND |
| P1036 | MAR | 0.5 | 4 | 4 | 0.25 | 0.25 | 8 | 32 | 8 | 8 | 32 | 2 | 1 | 13.2 ± 2 | 19.1 ± 1 |
a
WT, wild type.
b
Amp, ampicillin; Cip, ciprofloxacin; Chl, chloramphenicol; Ery, erythromycin; Kan, kanamycin; Tet, tetracycline; EtBr, ethidium bromide; AO, acridine orange; Deox, sodium deoxycholate; Cet, cetrimide; Tri, triclosan.
c
Micrograms of ciprofloxacin per nanogram of dry cells after 5 min of exposure (± standard deviation).
d
Fluorescence units of ethidium bromide after 5 min of exposure (± standard deviation).
e
ND, not determined.