FIG. 4.

Effects of trans-lactams on transcription. Cytoplasmic RNA was extracted from BT7H cells that either were left uninfected (U) or were infected with rVV NS3-5 or wild-type vaccinia virus (WT). Cells infected with rVV NS3-5 were also treated with compound 1a or 2 at the concentrations shown. Northern blot analysis was carried out using a biotinylated oligonucleotide probe to the NS5B region. All lanes contain 0.2 μg of cytoplasmic RNA, except for the positive control (P), which contains 10 ng of the NS3-5 RNA transcript.