FIG. 7.

Selection of the primary antibody for the rVV NS3-5 ELISA. (A) Commercially available murine monoclonal antibodies against HCV nonstructural proteins were assayed at 4 μg/ml against rVV NS3-5-infected BT7H cells (dark bars) and uninfected BT7H cells (light bars). Each bar represents the mean result for four (rVV NS3-5-infected cells) or three (uninfected cells) samples within the same experiment ± the standard error of the mean. Antibodies against the C-100 antigen (8958) and against NS5 (8808 and 1872) were selected for use in the ELISA (see Materials and Methods). (B) An example of an IC₅₀ curve for compound 1a using antibody 8958.