TABLE 2.
Comparison of trans-lactam potencies determined by the rVV NS3-5 and replicon ELISAsa
| Compound IDb | IC50 (μM) by the:
|
Biochemical potency (kobs/I [M−1 s−1])c | |
|---|---|---|---|
| Vaccinia virus ELISA | Replicon ELISA | ||
| 1d | 0.3 | <0.78 | 2,292 |
| 1c | 0.4 | 0.45 | 7,760 |
| 1i | 7.8 | 1.1 | 362 |
| 1j | 9 | 2.8 | 853 |
| 1n | 20.3 | 6.4 | 543 |
| 1a | 8.3 | 9.0 | 300 |
| 4 | >100 | 97 | 671 |
| 5 | 75 | >100 | 112 |
| 2 | >100 | >100 | 0 |
a
Results are ranked according to the data from the replicon ELISA.
b
ID, identification number.
c
Measured by a fluorogenic assay as described by Andrews et al. (1).