TABLE 2.
Effects of compounds on NADH dehydrogenasea
Test compound | Concn | % Inhibition |
---|---|---|
Triton X-100 | 0.005% | 50 |
Vancomycin | 100 μM | −1 |
Moenomycin | 1 μM | 5 |
Nisin | 100 μg/ml | 19 |
Tunicamycin | 30 μg/ml | 47 |
Penicillin | 100 μM | 13 |
Cefsulodin | 100 μM | 13 |
Cephalosporin C | 100 μM | 89 |
Cephalosporin C | 30 μM | 76 |
Compounds were tested on E. coli AMA1004 membranes under the conditions described in the text. For the experiment with cefsulodin and cephalosporin C, 0.5 μg of membrane protein was used; for all others, the quantity of protein used was 2 μg.