TABLE 1.
Target sequences for isoniazid resistance-directed oligonucleotide PCR and hybridization array
| Locus | Sequence type | Oligonucleotide name (sequence)a |
|---|---|---|
| katG 315 | Wild type | K315WTD20T (5′-CAGCGGCATCGAGGTCGTATTTTTTTTTTTTTTTTTTTTT-3′) |
| katG 315 | Mutant (AAC) | TOMK3159-AE10T (5′-CCAACGGCATCGAGGTCGTATTTTTTTTTT-3′) |
| katG 315 | Mutant (ACC) | TOMK3150-C10T (5′-CGGGATCACCACCGGCATCGTTTTTTTTTT-3′) |
| katG 463 | Arg | TOMK463WB5T (5′-GATCCGGGCATCGGGATTGACTTTTT-3′) |
| katG 463 | Leu | TOMK46399-TG10T (5′-GAGCCAGATCCTGGCATCGGGTTTTTTTTTT-3′) |
| oxyR-ahpC | Wild type | TOMAWT1A (5′-TTTTTTTTTTGCGACATTCCATCGTGCCG-3′) |
| oxyR-ahpC | Wild type | TOMAWT210T (5′-GTGCCGTGAAGTCGCTGTCAGTTTTTTTTTT-3′) |
| oxyR-ahpC | Wild type | TOMAWT320T (5′-TCAGGCAAAGGTGATATATCACACTTTTTTTTTTTTTTTTTTTT-3′) |
| inhA promoter | Wild type | TOMIWT10T (5′-GGCGAGACGATAGGTTGTCGGTTTTTTTTTT-3′) |
| inhA promoter | Mutant (−15T) | TOMIMUT110T (5′-GGCGAGATGATAGGTTGTCGGTTTTTTTTTT-3′) |
| inhA promoter | Mutant (−8A) | TOMIMUT210T (5′-GGCGAGACGATAGGATGTCGGTTTTTTTTTT-3′) |
a
Boldface indicates the specific nucleotides of interest.