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Expression of the hepcidin gene in various tissues of normal red sea bream (A) and red sea bream challenged with E. coli DH5α (B). Thirty-five cycles of amplification were used for panel A, and 25 cycles were used for panel B. −, fish injected with phosphate-buffered saline; +, fish injected with E. coli DH5α. 18S rRNA was used as the control.
