FIG 2.

ChPPK exhibited higher ATP regeneration activity from AMP than PPK12 in the presence of PPA. The courses of ATP generation (A), ADP generation (C), and AMP consumption (D) by ChPPK and PPK12 in the presence of PPA. Purified enzymes (5 µg/mL) of ChPPK or PPK12 were incubated with 1.6 g/L PPA and 2.25 mM AMP for the indicated periods. (B) The activities of purified ChPPK and purified PPK12. One unit of enzyme activity was given as the amount of enzyme that synthesized 1 µM ATP from AMP per minute during the initial minute.