TABLE 3.
Specific primers used for introducing site-directed mutations in ChPPK
| Primer | Oligonucleotide sequences (5′−3′) | Mutation |
|---|---|---|
| D77A-F | TCAGGCAATGGCTGCAGCAGGTAAAGAT | D77A |
| D77A-R | ACCTGCTGCAGCCATTGCCTGAAAAACA | |
| G80A-F | ATGCAGCAGCTAAAGATGGTACCGTT | G80A |
| G80A-R | ACCATCTTTAGCTGCTGCATCCATTG | |
| K81A-F | AGCAGGTGCAGATGGTACCGTTAAACATAT | K81A |
| K81A-R | TACCATCTGCACCTGCTGCATCCATTG | |
| D82A-F | AGGTAAAGCTGGTACCGTTAAACAT | D82A |
| D82A-R | ACGGTACCAGCTTTACCTGCTGCA | |
| F102A-F | TGACCAGCGCTAAAGTTCCGTCCAAAAT | F102A |
| F102A-R | ACGGAACTTTAGCGCTGGTCACTTTAACA | |
| K103A-F | ACCAGCTTTGCAGTTCCGTCCAAAATT | K103A |
| K103A-R | ACGGAACTGCAAAGCTGGTCACTTT | |
| V104A-F | AGCTTTAAAGCTCCGTCCAAAATTGAAC | V104A |
| V104A-R | TTGGACGGAGCTTTAAAGCTGGTCA | |
| P105A-F | TTTAAAGTTGCGTCCAAAATTGAACTGAG | P105A |
| P105A-R | ATTTTGGACGCAACTTTAAAGCTGG | |
| S106A-F | AAGTTCCGGCCAAAATTGAACTGAGT | S106A |
| S106A-R | TCAATTTTGGCCGGAACTTTAAAGCTGG | |
| I108A-F | TCCGTCCAAAGCTGAACTGAGTCATGATTA | I108A |
| I108A-R | ACTCAGTTCAGCTTTGGACGGAACTT | |
| S111A-F | TTGAACTGGCTCATGATTATCTGTGGC | S111A |
| S111A-R | TAATCATGAGCCAGTTCAATTTTGGACG | |
| R117A-F | TATCTGTGGGCTCATTATGTGGCAC | R117A |
| R117A-R | ACATAATGAGCCCACAGATAATCATGA | |
| R133A-F | TTTTTAACGCTAGCCATTATGAAAATGTGC | R133A |
| R133A-R | AATGGCTAGCGTTAAAAATACCAATTTCG | |
| E137A-F | AGCCATTATGCAAATGTGCTGGTTAC | E137A |
| E137A-R | AGCACATTTGCATAATGGCTACGGTTAAAAA | |
| N138A-F | ATTATGAAGCTGTGCTGGTTACCCGT | N138A |
| N138A-R | ACCAGCACAGCTTCATAATGGCTAC | |
| V141A-F | TGTGCTGGCTACCCGTGTACATCC | V141A |
| V141A-R | TACACGGGTAGCCAGCACATTTTCATA | |
| R208A-F | TTATTGAAGCTATCGAACTGGATACCAA | R208A |
| R208A-R | AGTTCGATAGCTTCAATAAAACGCTT | |
| R285STOP-F | ACCGTGAGCTAGGAACAGAAAGCGG | R285STOP |
| R285STOP-R | TTCTGTTCCTAGCTCACGGTCGGAAA |