TABLE 1.
Bacterial strains and plasmids used in this study
| Strain, plasmid, or cosmid | Characteristic or description | Source or reference |
|---|---|---|
| Bacterial strains | ||
| S. marcescens | ||
| UOC-67 | Type strain of S. marcescens | American Type Culture Collection |
| T-861 | Clinical isolate of S. marcescens | D. Hoban, Health Science Center, Winnipeg |
| UOC-67WL | Fluoroquinolone-resistant mutant derived from S. marcescens UOC-67 | 10 |
| UOC-67WLN | Fluoroquinolone-resistant mutant derived from S. marcescens UOC-67 | This study |
| UOC-67WLO | Fluoroquinolone-resistant mutant derived from S. marcescens UOC-67 | This study |
| E. coli | ||
| NM522 | Standard host strain for cloning | Promega |
| AG102MB | acrB-deletion mutant of E. coli AG100 | 4 |
| Plasmids or cosmids | ||
| pUC18 | Standard cloning vector, Apr | Stratagene |
| pDrive | PCR cloning vector, Kmr | QIAGEN |
| pRK7813 | Cosmid vector, Tcr | 7 |
| pRKO37 | pRK7813 containing 15-kb S. marcescens genomic insert | This study |
| pRKC51 | pRK7813 containing 20-kb S. marcescens genomic insert | This study |
| pUCAB | pUC18 containing S. marcescens sdeAB genes within a 5-kb EcoRV fragment | This study |
| pUCCDE | pUC18 containing S. marcescens sdeCDE genes within a 12-kb EcoRI/KpnI fragment | This study |