TABLE 1.

Oligonucleotide primers used for detection of β-lactamase genes

Primer	Tempa	Nucleotide sequence	GenBank accession no.	Nucleotide position	Expected amplicon size (bp)
CTX-M-2-S	58°C	5′-TTAATGATGACTCAGAGCATTC-3′	X92507	3-24	901
CTX-M-2-AS		5′-GATACCTCGCTCCATTTATTG-3′	X92507	884-904
CTX-M-9-S	50°C	5′-TAT TGG GAG TTT GAG ATG GT-3′	AF4546633.2	742-761	932
CTX-M-9-AS		5′-TCC TTC AAC TCA GCA AAA GT-3′	AF4546633.2	1655-1674
CTX-M-1-S	55°C	5′-CGT CAC GCT GTT GTT AGG AA-3′	AJ632119.1	180-209	780
CTX-M-1-AS		5′-ACG GCT TTC TGC CTT AGG TT-3′	AJ632119.1	941-960
TEM-S	50°C	5′-ATA AAA TTC TTG AAG ACG AAA-3′	AB103506	166-186	1,080
TEM-AS		5′-GAC AGT TAC CAA TGC TTA ATC-3′	AB103506	1225-1245
SHV-S	55°C	5′-TGG TTA TGG GTT ATA TTC GCC-3′	AY223863	166-186	865
SHV-AS		5′-GGT TAG CGT TGC CAG TGC T-3′	AY223863	1015-1031
OXA-1-S	55°C	5′-AGC CGT TAA AAT TAA GCC C-3′	AV162283.2	1052-1070	908
OXA-1-AS		5′-CTT GAT TGA AGG GTT GGG CG-3′	AV162283.2	1941-1960
CMY-1-S	60°C	5′-GAG CAG ACC CTG TTC GAG AT-3′	X92508	570-589	846
CMY-1-AS		5′-GAT TGG CCA GCA TGA CGA TG-3′	X92508	1397-1416
DHA-1-S	50°C	5′-GTT ACT CAC ACA CGG AAG GT-3′	AY205600	75-94	869
DHA-1-AS		5′-TTT TAT AGT AGC GGG TCT GG-3′	AY205600	925-944

^aAnnealing temperature used for PCR.