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. 2023 Nov 29;223(2):e202306107. doi: 10.1083/jcb.202306107

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© 2023 Li et al.

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Figure 3. — Dysregulation of RAB-8 leads to reduced resistance to multiple environmental stressors. (A) Confocal images of wild-type, rab-8(tm2526), and rabi-8(tm2518) animals after a 48-h incubation with fluorescent dye rho123. Compared with wild-type animals, the fluorescence intensity of rho123 increased sixfold in rabi-8(tm2518) and fivefold in rab-8(tm2526), respectively. Data are shown as mean ± SD (n = 18 each, six animals of each genotype sampled in three different unit regions of each intestine defined by a 100 × 100 [pixel²] box positioned at random). Statistical significance was determined using a one-way ANOVA followed by a post-hoc test (Dunn’s Multiple Comparison Test) for multiple comparisons. ***P < 0.001. Data distribution was assumed to be normal but this was not formally tested. Scale bars: 20 μm. (B) Compared with wild-type animals, the survival rate of rab-8(tm2526) and rabi-8(tm2518) mutants was reduced after 24 h of P. aeruginosa exposure. PGP-1 overexpression (OE) improved the survival rate of both mutants. (C) Compared with wild-type animals, the survival rate of rab-8(tm2526) and rabi-8(tm2518) mutants was reduced upon colchicine (2 mM) treatment. PGP-1 overexpression improved the survival rate of both mutants. (D) Mutations in rab-8 and rabi-8 reduced the resistance to arsenite (2 mM). Data are shown as mean ± SD. Statistical significance was determined using a one-way ANOVA followed by a post-hoc test (Dunn’s Multiple Comparison Test) for multiple comparisons. *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significance. Data distribution was assumed to be normal but this was not formally tested.