Skip to main content
. 2023 Nov 29;223(2):e202306107. doi: 10.1083/jcb.202306107

Figure S2.

Figure S2.

Some endolysosomal markers, the brood size, hatch rate, and several stress reporters are not affected in rabi-8 mutant animals. (A–C) The localization of fluorescence-tagged early endosome marker RAB-5 (A), basolateral recycling endosome marker RME-1 (B), and late endosome marker RAB-7 (C) were unaltered in rabi-8(tm2518) mutants. About one cell length of the intestine is shown in each panel. Scale bars: 10 μm. Yellow asterisks indicate intestinal lumen. (D and E) Mutation in rabi-8 had no effect on the brood size (D) or hatch rate (E), compared with wild-type animals. Three independent experiments were performed and data are shown as mean ± SD. (F–I) Confocal images of animals expressing Phsp-4::GFP (ER stress reporter) (F), Phsp-60::GFP (mitochondrial stress reporter) (G), Pgpdh-1::GFP (osmolyte accumulation) (H), and Pgst-4::GFP (detoxification) (I) in wild-type and rabi-8(tm2518) mutant animals. All the reporters displayed the similar expression and distribution upon rabi-8 depletion. Scale bars: 10 μm.