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. 2005 Apr;49(4):1441–1446. doi: 10.1128/AAC.49.4.1441-1446.2005

FIG. 3.

FIG. 3.

(A) Topo-I gene expression in LdRCPT.160. (A) Northern blot of L. donovani C1 and LdRCPT.160. The probe used corresponds to the SacI DNA fragment (1,069 bp) from the TOP1-like gene (LdTOP1A) of L. donovani. The LdTOP1A mRNA transcript is 3.4 kb long according to molecular size markers. RNA integrity was verified by ethidium bromide (EtBr) staining. This result is representative of one out of three experiments independently performed. (B) Topo-I DNA relaxation activity of LdRCPT.160. Supercoiled pBR322 DNA alone is shown in lane 1. Supercoiled pBR322 DNA in the presence of parasite extracts is represented in lanes 2 and 3. R, relaxed DNA form; SC, supercoiled DNA form. Results are representative of three experiments independently performed.