FIG. 2.

Immunoblot analysis of C. albicans extracts. About 60 μg of total protein was analyzed by immunoblotting with antibodies directed against Gas1p/Ggp1p and the ECL detection system (20). (A) Extracts from cells grown at 25°C in unbuffered YPD. (B) Extracts from cells grown at 25°C in YPD–50 mM HEPES. An extract (20 μg) of S. cerevisiae (Sc) was also loaded and deliberately overexposed. (C) Extracts from cells grown at 25°C in unbuffered YPD, filtered, and shifted at zero time to YPD pH 8 at 37°C. Arrows, immunoreactive bands detected by the anti-Ggp1p antibodies.